|
KMID : 1144820230290020081
|
|
대한의생명과학회지
2023 Volume.29 No. 2 p.81 ~ p.87
|
|
|
Evaluation of Proposed Diagnostic System for Detection of Pan-enterovirus Using Reverse Transcription Nested PCR from Water Environment
|
|
Lee Si-Won
Bae Kyung-Seon
Kim Jin-Ho
Park Ji-Hyun
Kim Ji-Hye
Park Ji-Yeon
Lee Kyung-Jin
Jeon Chae-Rin
Yoon Jeong-Ki
Lee Soo-Hyung
Park Eung-Roh
|
|
|
Abstract
|
|
|
|
Pan-Enterovirus (Pan-EV) infects millions of children and infants worldwide every year. As severe infections have recently been reported, the need for monitoring has consequently intensified. Pan-EV is a categorical name for waterborne enteroviruses belonging to the Picornaviridae family, and includes a wide range of pathogens including Coxsackievirus (CoxV), Echovirus (EcoV) and Enterovirus (EV). In this study, we proposed an optimal RT-nested PCR method for diagnosis of various types of Pan-EV in an aquatic environment and developed a positive control. Considering detection sensitivity, specific reaction, and final identification, one condition capable of amplifying 478 bp among the four candidates in the 1st round PCR (RT-PCR) and one condition in the 2nd round PCR (nested PCR) were selected. Through the detection of nucleic acids extracted from 123 groundwater samples and the detection sensitivity test based on artificial spiking in the sample, the methods are optimal for non-disinfected water samples such as groundwater. We developed a positive control for Pan-EV detection that can be amplified to different sizes under the two conditions. Accuracy could be further improved by testing for contamination from the control group. The method proposed in this study and the positive control developed are expected to be used in monitoring Pan-EV in aquatic environments including groundwater through future research using more samples.
|
|
|
KEYWORD
|
|
|
Groundwater, Pan-enterovirus, Pan-EV, Picornaviridae, RT-nested PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|